Hardware and settings guide

This guide will show how to setup Camera and Sample Mover in Breeze

Available Cameras and Sample Movers in Breeze

Open Settings

Start Breeze with the shortcut created after installation.
The Breeze start screen should look like this:

Breeze is organized into different views depending on the task at hand. Each view has a specific purpose as noted below each button.

Enter the Settings view by pressing the “Settings” button

Hardware

Select “Hardware”

Select Camera

Set the camera you would like to use in the “Primary Camera” drop down

Expand “Advanced” to see optional settings available for your camera (optional)

For example, select “Mirror line” to mirror the image

Under “Type” select your camera type

Breeze automatically connects to the selected Camera when pressing “Record” or “Analyse” button in the Record and Play view.

Expand advanced again to see updated options for your connected camera.
Set “Frame rate”. The number of frames per second.

If you are using “Offline Simulator Camera”, “Source” is available. The default source is “Automatic - Selected Study group”, which uses the first group with measurements from the selected Study.

Camera information is presented. In this example the “Offline Simulator Camera” is connected:

Sample Mover

Set the Sample Mover that you want to use in the “Selected Sample Mover” drop-down

Select “Serial port”

Advanced settings (Optional)

Different sample movers have different advanced options.

Press “Connect”

Breeze automatically connects to selected Sample Mover when pressing “Record” or Analyse button in the Record and Play view.

Sensor

Press the “Camera” switch. Shows bad or dead pixels

Integration Time

Adjust the “Integration Time” so that the highest value is between 80-90% (the background color changes to green) of the camera max signal, shown in the “Saturation ratio”.

Press “Camera”
Move “Position” slider to show white reference values on other pixels

Press “Min and max” to show minimum and maximum overall pixels

If “Integration Time” is set to high the white reference image will be saturated, which means that some signal data can be lost.

While recording images Breeze automatically checks the white reference for saturation or if the white reference signal is too low. If these problems occur it will be shown in the “Notifications” tab under “Measurements” in Record.

Available camera notifications:

• "Max signal exceeds the specified limit, possible saturation"

• "Max signal is lower than specified limit"

• "Uneven signal, possible defect lamp"

Focus

It’s important that the focus is set correctly for the lens before recording. Make sure that the focus strip position is the same as the highest point of the sample.

TIP Download a focus sheet here: https://prediktera.com/download/pdf/Prediktera_Breeze_Focus_Paper.pdf

“Field of view” is the width of the image you can see in focus, specified in millimeters.
It’s important that the “Field of view” is correct since it will be used to calculate the speed of the Sample mover. If the “Field of view” is incorrect it will result in non-square pixels.

“Field of view” is also used in the conversion of pixels into mm units. This is, for example, used in spatial descriptors.

Press “Camera” and press “Focus grid”

You can make use of the “Focus rate” value to get the best focus. Adjust manually the lens and until you reach the highest focus rate and the lines are sharp.

The “Line plot” can also help you to set the focus.

It’s optional to select different bands.

Press “Scan” and measure a round object to make sure that the “Field of view” is correctly set.

Measurement

Set “Measurement Length”.

This is how long the image scan will be in millimeters, frames or seconds.

OPTIONAL The “Reference length” is how many frames (lines) it will scan when taking the white and dark reference.

OPTIONAL Breeze can store “White reference” and “Dark reference” images in memory during the Breeze session. The time, in seconds, that the references are stored is specified in the two input fields. When the time specified has expired Breeze will automatically take new references. If the values are 0 (default), it will take a new white and dark reference for every new scan.

Example below,the reference will be stored for two hour before it will take a new reference.

You have now completed the camera and sample mover setup! Switch to record mode to start scanning your samples.