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Intensity

Segment objects from measurements using intensities in the different bands/wavelengths

Parameters

Convert to

If the spectral data should be converted before applying the expression. Learn more inChanging spectra. No conversion will be made if the data already is the same format.

Expression

Write expression. Band numbers are denoted b[N] e.g. b1 and wavelengths are denoted w[nm] e.g. w1200. The following comparators can be used for the expression:

Operators than can be used expressions include the data operators wNNN and bMMM for referring to wavelength bands, the range operator : used for averaging data, standard arithmetic (+,-,/,* …) and comparison operators (=,>,< …) as well as some mathematical functions ( …) and constants ( ).

Breeze does not validate the provided expression until you click Apply changes to apply it to some data.

Data Operator

Description

wNNN

Wavelength lookup operator that finds the wavelength band closest to the provided number NNN. This means NNN need not match exactly to find data.

A setting controls how far off a wavelength is allowed to be to be considered a match. If there isn’t matching data an error is displayed when applying the workflow to data. Learn more in Wavelength matching.

Example of this syntax: w700 or w1714.

bMMM

Band index operator. MMM represent the one-based index of a wavelength band. For example b1 is the first band, and b20 is the twentieth.

If the index MMM does not exist, Breeze displays an error message.

:

Average range operator that returns the average value for a range of wavelength bands.

For example: w1200:w1500 yields average value of all data points between wavelength 1200 nm and 1500 nm. b1:b2 yields average values of the first two bands.
Expand to see all available operators

Arithmetic Operator

Description

-

Subtract

+

Add

/

Divide

*

Multiply

%

Modulo

^

Raised to a power

Comparison Operator

Description

=

Equal to

|

OR

TRUE if any of the conditions separated by OR is TRUE

&

AND

TRUE if all the conditions separated by AND is TRUE

!=

<>

Not equal to

<

Less than

<=

Less than or equal to

>

Greater than

>=

Greater than or equal to

Function operator

Description

SQRT(N)

SIN(N)

COS(N)

EXP(N)

LOG(N)

LOG10(N)

AVG(N)

ROUND(N)

Constants

Description

TRUE

Always evaluates to TRUE

FALSE

Always evaluates to FALSE

INF

token value

PI

approximated to

Variable names that can be used in the expression:

  • MinValue

  • MaxValue

  • AvarageValue

Pretreatments

In top-down applied order ⬇️ , meaning Savitzky-Golay is applied first then Derivative and so on.

  • Savitzky-Golay

  • Derivative

  • SNV (Standard Normal Variate)

  • Logarithm

  • Center

  • UV (Unit Variance) scaling

For more on each type of pretreatment see: Pretreatments

Min area

The minimum number of pixels for an object to be included.

Max area

The maximum number of pixels for an object to be included.

If 0 no maximum area is defined.

Object filter

Use an expression to further exclude unwanted objects based on shape.

Properties that can be used for the Expression:

  • Area

  • Length

  • Width

  • Circumference

  • Regularity

  • Roundness

  • Angle

  • D1

  • D2

  • X

  • Y

  • MaxBorderDistance

  • BoundingBoxArea

For details on each available property see: Object properties Details

Shrink

Takes away x numbers of pixels at the borders of the objects included in images.

Separate

The Separate parameter defines how pixels that pass your segmentation threshold are grouped into distinct object samples.

Pixel Connectivity Rule

Breeze uses 8-connectivity. Pixels are considered connected if they touch horizontally, vertically, or diagonally on a single corner.

Parameter Options

1. Normal

  • Behavior: Standard connected-component grouping.

  • Result: Pixels touching by an edge or corner form a single object. Physically isolated clusters form separate objects.

2. Separate adjacent objects

  • Behavior: Applies a distance transform to find the inner centers (geometric peaks) of pixel clusters, then splits them using a watershed-style growth algorithm.

  • Best Used For: Round or spherical objects that are touching or overlapping.

  • Limitation: Elongated, branched, or irregular shapes may be incorrectly split into multiple objects because the algorithm detects multiple internal peaks within a single physical item.

3. Merge all objects into one

  • Behavior: Disregards spacing; combines all valid pixels across the entire image.

  • Result: Evaluates the entire frame as one single object for collective regional analysis.

4. Merge all objects per row

  • Behavior: Groups detected objects based on the spatial grid of their vertical center points ($Y$-coordinates).

  • Result: All objects residing within the same horizontal row lane are merged into a single object.

5. Merge all objects per column

  • Behavior: Groups detected objects based on the spatial grid of their horizontal center points ($X$-coordinates).

  • Result: All objects residing within the same vertical column lane are merged into a single object.

Only visible when applicable

Link output objects from two or more segmentations to top segmentation. Descriptors can then be added to the common object output and will be calculated for objects from all segmentations.

image-20230303-095316.png
Descriptors after object will be calculated for all three segmentations (Sample1, Sample2 and Sample3)

The segmentations must be at same level to be available for linking.

Example

Using band numbers

Expression: (b10 > 1.5 | b15 < 2) & b20 <= 1.2

Using wavelengths

Expression: (w980 > 1.5 | w1024 < 2) & w1200 <= 1.2

Use variables for the expression

Expression: w980 > AvarageValue