Model

Select a PCA model to separate the background and the sample in the images.

Parameters

Model

Select a PCA model to remove unwanted parts of the image

Min area

The minimum number of pixels for an object to be included.

Max area

The maximum number of pixels for an object to be included.

If = 0 no maximum area is defined.

Object filter

Use an expression to further exclude unwanted objects based on shape.

Properties that can be used for the Expression:

• Area

• Length

• Width

• Circumference

• Regularity

• Roundness

• Angle

• D1

• D2

• X

• Y

• MaxBorderDistance

• BoundingBoxArea

For details on each available property see: Object properties Details

Shrink

Takes away x numbers of pixels at the borders of the objects included in images.

Separate

One of the options below

• Normal

  • Can have both separated and combined objects.

• Separate adjacent objects

  • All objects are defined separately.

• Merge all objects into one

  • All objects are defined as one.

• Merge all objects per row

  • All objects per row segmentation are defined as one.

• Merge all objects per column

  • All objects per column segmentation are defined as one.

Inverse

✅ Includes the opposite of the sample specified in the PCA.

⬜ Includes the sample specified from the PCA.

Link

Only visible when applicable

Link output objects from two or more segmentations to top segmentation. Descriptors can then be added to the common object output and will be calculated for objects from all segmentations.

The segmentations must be at same level to be available for linking.